Contamination of nanoparticles in determining immunotoxic and inflammatory effects: revisiting the basic concepts of nanotoxicology
Programme of the Session
- Nanomaterials hoax scientists: nanosafety research on the right track?
Harald F. Krug
International research cooperation manager, Empa - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland
- The hidden toxin. Is bacterial lipopolysaccharide contamination responsible for nanomaterial toxicity?
Diana Boraschi, Yang Li, Paola Italiani
Institute of Protein Biochemistry, National Research Council, Napoli, Italy
- Allergen-carrying nanoparticles induce novel immunotoxic responses
Isabella Radauer-Preiml, Mark Geppert, Martin Himly, Albert Duschl
Molecular Biology, University of Salzburg, Salzburg, Austria
- Endotoxin limits and in vivo immunosafety testing for nanomedicines: what you need to remember
Pharmaceutical Technology and Biopharmacy, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany
- Nanoparticle interaction with biomolecules: how it shapes the nano-effects on immunity
Vall Hebron Institute of Research - Catalan Institute of Nanotechnology, Barcelona, Spain
Nanotoxicology implies studying the effects of nanomaterials on biological systems in in vivo or in vitro models. Most studies however fail to acknowledge that nanomaterials have highly reactive surface that can adsorb all kinds of molecules during synthesis, handling and experimental procedures. Such molecules encompass sterilisation-resistant ubiquitous contaminants such as bacterial endotoxin, but also other microbial agents, allergens, etc. Thus, the nanomaterial may serve as carrier and change the immunogenic or immunotoxic capacity of the carried agents, and vice-versa the agent may change the type of interaction of the nanomaterial with cells. This workshop intends to raise awareness on the issue of contamination of nanomaterials with biologically active bystander substances and on the possible biological and toxicological consequences of such contamination. This awareness should help us devising assays and procedures for improving the reliability of nanotoxicological testing for regulatory purposes.